Three-dimensional reconstruction of a skeletal muscle cell from optical sections

T. R. Gowrishankar; Raphael C. Lee M.D.; Chin-Tu Chen

doi:10.1117/12.148629

29 July 1993 Three-dimensional reconstruction of a skeletal muscle cell from optical sections

T. R. Gowrishankar, Raphael C. Lee M.D., Chin-Tu Chen

Proceedings Volume 1905, Biomedical Image Processing and Biomedical Visualization; (1993) https://doi.org/10.1117/12.148629
Event: IS&T/SPIE's Symposium on Electronic Imaging: Science and Technology, 1993, San Jose, CA, United States

Abstract

Fluorescence microscopy assay of cell membranes is a practical way of studying the primary mechanisms of muscle cell damage in electrical injury. The three-dimensional distribution of pores in an electric field can be measured by 3-D imaging of the distribution of a fluorescent potentiometric dye. A confocal microscope with a variable-width slit detectors, a high frequency non-mechanical scanning system and multiple-line laser illumination is highly suitable for the optical measurement of membrane potential. In this paper, the characteristics of such a confocal microscope in terms of its 3-D optical transfer function (OTF) measured using fluorescent beads is presented. 3-D reconstruction of a skeletal muscle cell stained with the potentiometric dye, di-8-ANEPPS from optical sections is also demonstrated.

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T. R. Gowrishankar, Raphael C. Lee M.D., and Chin-Tu Chen "Three-dimensional reconstruction of a skeletal muscle cell from optical sections", Proc. SPIE 1905, Biomedical Image Processing and Biomedical Visualization, (29 July 1993); https://doi.org/10.1117/12.148629

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