Determination of the energy transfer rate in photosynthetic systems by means of time-resolved spectroscopy

Leonas Valkunas; Gediminas Trinkunas

doi:10.1117/12.146153

17 June 1993 Determination of the energy transfer rate in photosynthetic systems by means of time-resolved spectroscopy

Leonas Valkunas, Gediminas Trinkunas

Proceedings Volume 1921, Laser Spectroscopy of Biomolecules; (1993) https://doi.org/10.1117/12.146153
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

This report addresses the energy migration problem in the light-harvesting antenna systems of photosynthetic objects. Both linear and nonlinear relaxation cases are discussed. In the linear case, the spectral inhomogeneity is taken into account by averaging the Master equation for the excitation over the spectrally inhomogeneous distribution of pigment molecules. That reformulates the Master equation as the excitation diffusion in the energy space. The stationary solution of this equation enables the determination of the Stokes shift dependence on homogeneous and inhomogeneous spectral bandwidths. The nonlinear relaxation is considered at the singlet-singlet annihilation conditions. The analysis of the fluorescence quantum yield, the excitation delay kinetics as well as the changes of spectral properties of the object under consideration as a function of the excitation intensity, demonstrates the possibility to determine the energy migration parameters. An important conclusion of this analysis is that the real pulse-shape of the excitation as well as the population of higher excited states during the nonlinear annihilation have to be taken into account.

Citation Download Citation

Leonas Valkunas and Gediminas Trinkunas "Determination of the energy transfer rate in photosynthetic systems by means of time-resolved spectroscopy", Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); https://doi.org/10.1117/12.146153

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