In vivo pharmacokinetic study of two fluorescein derivatives by fluorescence spectroscopy

Sylvie Soulie-Begu; Jean-Marie Devoisselle; Serge R. Mordon

doi:10.1117/12.228881

27 December 1995 In-vivo pharmacokinetic study of two fluorescein derivatives by fluorescence spectroscopy

Sylvie Soulie-Begu, Jean-Marie Devoisselle, Serge R. Mordon

Proceedings Volume 2627, Optical Biopsies; (1995) https://doi.org/10.1117/12.228881
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

We have already demonstrated the ability of fluorescence spectroscopy and imaging to measure the pH of superficial tissues using pH sensitive fluorescent probes. The purpose of this study was to investigate the in vivo behavior of such fluorescent probes. We report the monitoring of tissue fluorescence after injection of two fluorescein derivatives (carboxyfluorescein and biscarboxyethyl-carboxyfluorescein). The in vivo study was performed on anaesthetized adult Wistar rats. After laparotomy, CF or BCECF solution was injected into the penial vein. Fluorescence spectra were recorded during one hour using an optical multichannel analyzer coupled to a CCD camera. Fiber optic was placed alternatively on the liver area or on the skin. Blood samples were collected and fluorescence was measured in vitro. A clear linear relationship between dose and fluorescence intensity was found in liver for these fluorescent markers. Concerning spectral characteristics, it was found that CF and BCECF spectra show a shift compared to in vivo maximum emission peak and BCECF emission peak was different when recorded in the liver and in the skin. Differences of kinetic profiles are also observed between CF and BCECF. The BCECF derivative displays a fluorescence peak in the liver two minutes after injection, while CF fluorescence peak is observed seven minutes after injection. Clearance of skin fluorescence is slower than the plasmatic one indicating that dye elimination in superficial blood vessels does not follow the same pharmacokinetic behavior. Based on these preliminary findings, fluorescence spectroscopy appears as a tool in pharmacokinetic study in situ and in vivo.

Citation Download Citation

Sylvie Soulie-Begu, Jean-Marie Devoisselle, and Serge R. Mordon "In-vivo pharmacokinetic study of two fluorescein derivatives by fluorescence spectroscopy", Proc. SPIE 2627, Optical Biopsies, (27 December 1995); https://doi.org/10.1117/12.228881

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available