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Proceedings Article

Fluorescence microtomography: multiangle image acquisition and 3D digital reconstruction

[+] Author Affiliations
Carol J. Cogswell, Kieran G. Larkin, Hanno U. Klemm

Univ. of Sydney (Australia)

Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, 109 (April 10, 1996); doi:10.1117/12.237467
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From Conference Volume 2655

  • Three-Dimensional Microscopy: Image Acquisition and Processing III
  • Carol J. Cogswell; Gordon S. Kino; Tony Wilson
  • San Jose, CA | January 28, 1996

abstract

We have developed a prototype fluorescence microscope which, using tomographic image acquisition and reconstruction techniques, can automatically combine conventional and/or confocal image stacks taken at a number of orientations into a single, very-high-resolution 3D image. We use the term `microtomography' in a broad sense to denote digital image reconstruction from multiple imaging operations which are not necessarily projections. Our system holds a biological specimen inside a thin capillary tube which is rotatable over a 360 degree range beneath an immersion objective. 3D fluorescence image data volumes are acquired by frame-grabbing a through-focus series of 2D images at each angle of rotation. Digital reconstruction of the multi-angle data volumes produces a single very-high-resolution 3D image and involves algorithms which perform rotation, interpolation, alignment and normalization operations in frequency (Fourier) space.

© (1996) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Carol J. Cogswell ; Kieran G. Larkin and Hanno U. Klemm
"Fluorescence microtomography: multiangle image acquisition and 3D digital reconstruction", Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, 109 (April 10, 1996); doi:10.1117/12.237467; http://dx.doi.org/10.1117/12.237467


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