Analysis of erythrocyte flexibility by means of laser diffraction: rigidification due to defined shearing

Rainer Bayer; Guido Wolf

doi:10.1117/12.146478

2 June 1993 Analysis of erythrocyte flexibility by means of laser diffraction: rigidification due to defined shearing

Rainer Bayer, Guido Wolf

Proceedings Volume 1981, Optical Methods of Biomedical Diagnostics and Therapy; (1993) https://doi.org/10.1117/12.146478
Event: Radiofrequency and Optical Methods of Biomedical Diagnostics and Therapy, 1992, Saratov, Russian Federation

Abstract

A newly developed system to determine red blood cell flexibility is presented. It combines ektacytometry (laser diffraction) with image analysis. Laser light is passed through erythrocyte suspensions which are sheared in a Cuette like viscosimeter. The laser diffraction patterns are photographed by a CCD camera, and further analyzed by a computer. Flexibility is characterized by the quotient of minor and major axes of isointensity lines of the elliptically transformed diffraction patterns. The variation coefficient of the measurement is less than 1%.

Citation Download Citation

Rainer Bayer and Guido Wolf "Analysis of erythrocyte flexibility by means of laser diffraction: rigidification due to defined shearing", Proc. SPIE 1981, Optical Methods of Biomedical Diagnostics and Therapy, (2 June 1993); https://doi.org/10.1117/12.146478

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