Cykotine mRNA expression in mouse retina after laser injury by reverse transcriptase-polymerase chain reaction (RT-PCR)

Steven T. Schuschereba; Phillip D. Bowman; Veronica Ujimore; Stephen W. Hoxie; Jose M. Pizarro; Michael E. Cross; David J. Lund

doi:10.1117/12.237503

1 April 1996 Cykotine mRNA expression in mouse retina after laser injury by reverse transcriptase-polymerase chain reaction (RT-PCR)

Steven T. Schuschereba, Phillip D. Bowman, Veronica Ujimore, Stephen W. Hoxie, Jose M. Pizarro, Michael E. Cross, David J. Lund

Author Affiliations +

Proceedings Volume 2674, Laser-Inflicted Eye Injuries: Epidemiology, Prevention, and Treatment; (1996) https://doi.org/10.1117/12.237503
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

The purpose of this study was to identify cytokines produced by the retina after laser injury. With the aid of a scanning laser ophthalmoscope (SLO), right eyes of mice received lesions from a continuous wave argon laser. Left eyes served as unirradiated controls. At 2, 4, 6, 12, 24, and 48 hr after laser irradiation groups of 3 mice were euthanized and retinas fixed for histology or isolated for RNA. Messenger RNA (mRNA) was reverse-transcribed into complementary DNA (cDNA) and subjected to polymerase chain reaction for the following cytokines: tumor necrosis factor-(alpha) (TNF-(alpha) ), interleukin-1(alpha) /(Beta) (IL- 1(alpha) /(Beta) ), interleukin-6 (IL-6), transforming growth factor-(Beta) 1 (TGF- (Beta) 1), macrophage colony stimulating factor (M-CSF), inducible nitric oxide synthase (iNOS), and glyceraldehyde 3-phosphate dehydrogenase (G3PDH). Histologically, lesions were confined to the photoreceptors, retinal pigment epithelium, and choroid. In laser-injured retinas, mRNA levels were elevated for IL-1(alpha) , TGF-(Beta) 1, iNOS, and G3PDH, but not TNF-(alpha) , IL-1(Beta) , or IL-6. It appears that the retina, in response to laser injury, upregulates a select number of cytokines in a time-course dependent fashion.

Citation Download Citation

Steven T. Schuschereba, Phillip D. Bowman, Veronica Ujimore, Stephen W. Hoxie, Jose M. Pizarro, Michael E. Cross, and David J. Lund "Cykotine mRNA expression in mouse retina after laser injury by reverse transcriptase-polymerase chain reaction (RT-PCR)", Proc. SPIE 2674, Laser-Inflicted Eye Injuries: Epidemiology, Prevention, and Treatment, (1 April 1996); https://doi.org/10.1117/12.237503

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available