In vivo fluorescence spectroscopy of the gastrointestinal tract under multiple wavelength excitation

Haishan Zeng; Alan Weiss; Nick MacKinnon; Richard W. Cline; Calum E. MacAulay

doi:10.1117/12.260812

6 December 1996 In vivo fluorescence spectroscopy of the gastrointestinal tract under multiple wavelength excitation

Haishan Zeng, Alan Weiss, Nick MacKinnon, Richard W. Cline, Calum E. MacAulay

Proceedings Volume 2926, Optical Biopsies and Microscopic Techniques; (1996) https://doi.org/10.1117/12.260812
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

Recent spectroscopy studies have shown that tissue autofluorescence properties are excitation wavelength dependent. In this work, we developed a nitrogen dye laser - OMA system capable of quickly measuring tissue autofluorescence spectra in vivo under multiple wavelength excitation. The system consists of a nitrogen dye laser with stepper motor for wavelength selection, an OMA with gated intensified detector, and a PC computer. A bifurcated fiber optic bundle is used to conduct the excitation laser light and to collect the fluorescence light. Gating electronics are used to achieve a high signal to noise ratio, allowing fluorescence measurements to be performed with ambient light on or under white light illumination during endoscopy. Excitation wavelength changes are performed automatically and quickly by the stepper motor which is controlled by the OMA PIA port. The system has been used to acquire spectra directly from the tissue of interest in patients undergoing gastroscopic and colonoscopic examination. The time needed to switch from one excitation wavelength to the next excitation wavelength is about one second.

Citation Download Citation

Haishan Zeng, Alan Weiss, Nick MacKinnon, Richard W. Cline, and Calum E. MacAulay "In vivo fluorescence spectroscopy of the gastrointestinal tract under multiple wavelength excitation", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); https://doi.org/10.1117/12.260812

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