0

Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Comparative study of three-dimensional localization accuracy in conventional, confocal laser scanning and axial tomographic fluorescence light microscopy

[+] Author Affiliations
Joachim Bradl, Bernd Rinke, Arif Esa, Peter Edelmann, Hans Krieger, Bernhard Schneider, Michael Hausmann, Christoph G. Cremer

Ruprecht-Karls-Univ. Heidelberg (Germany)

Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, 201 (December 6, 1996); doi:10.1117/12.260797
Text Size: A A A
From Conference Volume 2926

  • Optical Biopsies and Microscopic Techniques
  • Irving J. Bigio; Warren S. Grundfest; Herbert Schneckenburger; Katarina Svanberg; Pierre M. Viallet
  • Vienna, Austria | September 07, 1996

abstract

For many biological applications, precise and accurate 3D object localizations and 3D-distance measurements are necessary. Point spread functions of artificial objects of subwavelength dimensions have been measured in order to characterize the image forming properties as well as to localize extended objects in both conventional and confocal florescence light microscopy with and without the axial tomographic technique. With the axial tomographic technique it is possible to tilt the object in such a way, that substructures are located in the same focal plane. The distance of two points measured under this optimal perspective fits best to the real 3D-distance. In this case, optical sectioning is unnecessary, if only distance measurements have to be performed.

© (1996) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Joachim Bradl ; Bernd Rinke ; Arif Esa ; Peter Edelmann ; Hans Krieger, et al.
"Comparative study of three-dimensional localization accuracy in conventional, confocal laser scanning and axial tomographic fluorescence light microscopy", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, 201 (December 6, 1996); doi:10.1117/12.260797; http://dx.doi.org/10.1117/12.260797


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
 

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement


Buy this article ($18 for members, $25 for non-members).
Sign In