Confocal fluorescence microscopy, microspectrofluorimetry, and modeling studies of laser-induced fluorescence endoscopy (LIFE) of human colon tissue

Ralph Sebastian DaCosta; Lothar D. Lilge; James Kost; Maria Cirroco; Shirley Hassaram; Norman E. Marcon M.D.; Brian C. Wilson

doi:10.1117/12.275463

16 June 1997 Confocal fluorescence microscopy, microspectrofluorimetry, and modeling studies of laser-induced fluorescence endoscopy (LIFE) of human colon tissue

Ralph Sebastian DaCosta, Lothar D. Lilge, James Kost, Maria Cirroco, Shirley Hassaram, Norman E. Marcon M.D., Brian C. Wilson

Author Affiliations +

Proceedings Volume 2975, Laser-Tissue Interaction VIII; (1997) https://doi.org/10.1117/12.275463
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

In vivo LIF spectroscopy and imaging, using 442 nm exc. light, delineates normal form adenomatous colonic tissue. This study investigates the microscopic origin of the observed tissue autofluorescence differences using confocal fluorescence microscopy, histological analysis, confocal microspectrofluorimetry and Monte Carlo simulation. A multilayered tissue model is developed for normal, flat and polypoid adenomas, based on: (1) tissue architecture, (2) optical properties, (3) fluorescence distribution, and (4) illumination/detection geometries. Major differences were found in fluorescence intensity and spectral lineshape between normal and adenomatous colon samples. Fluorescence spectral composition of normal and adenomatous colon autofluorescence is tissue depth-dependent for normal, premalignant, and malignant tissues. Mathematical modeling suggests that LIF measurements are primarily sensitive to changes in tissue morphology specific to pathological progression, including mucosal thickening, alteration in mucosal tissue constituents, redistribution of submucosal collagen and significantly increased blood volume.

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Ralph Sebastian DaCosta, Lothar D. Lilge, James Kost, Maria Cirroco, Shirley Hassaram, Norman E. Marcon M.D., and Brian C. Wilson "Confocal fluorescence microscopy, microspectrofluorimetry, and modeling studies of laser-induced fluorescence endoscopy (LIFE) of human colon tissue", Proc. SPIE 2975, Laser-Tissue Interaction VIII, (16 June 1997); https://doi.org/10.1117/12.275463

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