Virus structure using the computer-aided phase microscope Airyscan

Vladimir P. Tychinsky; Nikolai V. Kaverin; Elena V. Perevedentseva; Tatiana V. Vyshenskaia; Georgy E. Kufal

doi:10.1117/12.271268

10 April 1997 Virus structure using the computer-aided phase microscope Airyscan

Vladimir P. Tychinsky, Nikolai V. Kaverin, Elena V. Perevedentseva, Tatiana V. Vyshenskaia, Georgy E. Kufal

Proceedings Volume 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV; (1997) https://doi.org/10.1117/12.271268
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Investigation of features and functions of some small biological objects (smaller than 500 nm), in particular, viruses, with conventional optical microscopy is practically impossible. Usually their images are obtained with methods of scanning electron microscopy (SEM), which precludes work with samples in a native state. We obtained images of different viruses including influenza A virus in native state with computer-aided phase microscope (CPM) Airyscan, in which an He-Ne laser is used as a light source. The main purpose of this work was to show the possibility to obtain adequate structure images of influenza viruses with diameter about 100 nm in conditions quite close to native and to investigate different stages of influenza virus budding. We suppose that these results may be considered as a basis for further studies of cell-virus interaction.

Citation Download Citation

Vladimir P. Tychinsky, Nikolai V. Kaverin, Elena V. Perevedentseva, Tatiana V. Vyshenskaia, and Georgy E. Kufal "Virus structure using the computer-aided phase microscope Airyscan", Proc. SPIE 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV, (10 April 1997); https://doi.org/10.1117/12.271268

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