Three-dimensional imaging through millimeter-thick tissue specimens

Michael G. Jones; Michael Halliwell; David R. Bull; Jack D. Davies; Catherine N. Chinyama; Peter N. T. Wells

doi:10.1117/12.271263

10 April 1997 Three-dimensional imaging through millimeter-thick tissue specimens

Michael G. Jones, Michael Halliwell, David R. Bull, Jack D. Davies, Catherine N. Chinyama, Peter N. T. Wells

Proceedings Volume 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV; (1997) https://doi.org/10.1117/12.271263
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

A 780 nm stage-scanned confocal transmission microscope using compact disk (CD) player optics has been constructed and used to study test objects (including scattering effects) and breast tissue specimens (25-1500 micrometer thick). Confocal alignment in transmission is achieved using the CD optical mechanism moving coils to scan the objective lens until intensity peaks; the specimen is present but motionless. Intensity variation as a 3D function of mis- alignment of the two foci may allow estimation of optic deterioration caused by the specimen; appropriate confocal apertures and sample spacings can then be selected. Experimental measurements have been supplemented by 2D Monte-Carlo modeling of photon transport for a scattering- limited confocal transmission system. Modeling has characterized: transmission as a function of focal depth in a slab; contrast produced by a range of embedded spherical target radii; and optical mis-alignment. Poor dye perfusion within thick stained specimens results in little visible internal structure. This may be improved using partially phase dependent imaging (approximately equal to split- detector), sensitive to specimen refractive index variations.

Citation Download Citation

Michael G. Jones, Michael Halliwell, David R. Bull, Jack D. Davies, Catherine N. Chinyama, and Peter N. T. Wells "Three-dimensional imaging through millimeter-thick tissue specimens", Proc. SPIE 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV, (10 April 1997); https://doi.org/10.1117/12.271263

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