Compact upconverting phosphor detection system for wick assays

Haris Riris; Jan E. Van der Laan; David E. Cooper; Karen M. Nashold; Clinton B. Carlisle; Luke V. Schneider

doi:10.1117/12.274359

22 May 1997 Compact upconverting phosphor detection system for wick assays

Haris Riris, Jan E. Van der Laan, David E. Cooper, Karen M. Nashold, Clinton B. Carlisle, Luke V. Schneider

Proceedings Volume 2985, Ultrasensitive Biochemical Diagnostics II; (1997) https://doi.org/10.1117/12.274359
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

We describe an integrated detection system based on upconverting phosphor particles bound to capture sites on the inside surfaces of rectangular wick capillaries. This deice can be used with either antibody or nucleic acid to detect specific micro-organisms. The system uses a high- power, 980 nm, semiconductor diode laser to illuminate 200 X 300 X 20 micrometers capture surfaces. The rectangular capillary wicks are held in a tray that is inserted into the detection system, positioning the capture surface at the object plane of the optical system. Phosphorescent light emitted from the capture surface is collected by a high numerical aperture microscope objective and directed through a series of filters onto either a CCD camera or a photomultiplier. A combination of band-reject filters attenuates the 980 nm laser excitation light and its harmonic at 490 nm, and a tunable liquid crystal filter provides for rapid scanning from 400 to 750 nm. The data acquisition and control is controlled by a laptop PC with a custom GUI interface developed using LabWindows/CVI. The system can detect a single phosphor particle bound to a capture surface.

Citation Download Citation

Haris Riris, Jan E. Van der Laan, David E. Cooper, Karen M. Nashold, Clinton B. Carlisle, and Luke V. Schneider "Compact upconverting phosphor detection system for wick assays", Proc. SPIE 2985, Ultrasensitive Biochemical Diagnostics II, (22 May 1997); https://doi.org/10.1117/12.274359

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available