Targeted detection of murine colonic dysplasia in vivo with flexible multispectral scanning fiber endoscopy

Bishnu P. Joshi; Sharon J. Miller; Cameron Lee; Adam Gustad; Eric J. Seibel; Thomas D. Wang M.D.

doi:10.1117/12.906465

2 March 2012 Targeted detection of murine colonic dysplasia in vivo with flexible multispectral scanning fiber endoscopy

Bishnu P. Joshi, Sharon J. Miller, Cameron Lee, Adam Gustad, Eric J. Seibel, Thomas D. Wang M.D.

Author Affiliations +

Proceedings Volume 8217, Endoscopic Microscopy VII; 82170J (2012) https://doi.org/10.1117/12.906465
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We demonstrate a multi-spectral scanning fiber endoscope (SFE) that collects fluorescence images in vivo from three target peptides that bind specifically to murine colonic adenomas. This ultrathin endoscope was demonstrated in a genetically engineered mouse model of spontaneous colorectal adenomas based on somatic Apc (adenomatous polyposis coli) gene inactivation. The SFE delivers excitation at 440, 532, 635 nm with <2 mW per channel. The target 7-mer peptides were conjugated to visible organic dyes, including 7-Diethylaminocoumarin-3-carboxylic acid (DEAC) (λ_ex=432 nm, λ_em=472 nm), 5-Carboxytetramethylrhodamine (5-TAMRA) (λ_ex=535 nm, λ_em=568 nm), and CF-633 (λ_ex=633 nm, λ_em=650 nm). Target peptides were first validated using techniques of pfu counting, flow cytometry and previously established methods of fluorescence endoscopy. Peptides were applied individually or in combination and detected with fluorescence imaging. The ability to image multiple channels of fluorescence concurrently was successful for all three channels in vitro, while two channels were resolved simultaneously in vivo. Selective binding of the peptide was evident to adenomas and not to adjacent normal-appearing mucosa. Multispectral wide-field fluorescence detection using the SFE is achievable, and this technology has potential to advance early cancer detection and image-guided therapy in human patients by simultaneously visualizing multiple over expressed molecular targets unique to dysplasia.

Citation Download Citation

Bishnu P. Joshi, Sharon J. Miller, Cameron Lee, Adam Gustad, Eric J. Seibel, and Thomas D. Wang M.D. "Targeted detection of murine colonic dysplasia in vivo with flexible multispectral scanning fiber endoscopy", Proc. SPIE 8217, Endoscopic Microscopy VII, 82170J (2 March 2012); https://doi.org/10.1117/12.906465

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