Video-based analysis of the rotational behaviour of rod-shaped, self-propelled bacteria in holographic optical tweezers

Lena Dewenter; Christina Alpmann; Mike Woerdemann; Cornelia Denz

doi:10.1117/12.922449

8 May 2012 Video-based analysis of the rotational behaviour of rod-shaped, self-propelled bacteria in holographic optical tweezers

Lena Dewenter, Christina Alpmann, Mike Woerdemann, Cornelia Denz

Author Affiliations +

Proceedings Volume 8427, Biophotonics: Photonic Solutions for Better Health Care III; 84270N (2012) https://doi.org/10.1117/12.922449
Event: SPIE Photonics Europe, 2012, Brussels, Belgium

Abstract

Utilising the versatility of holographic optical tweezers and high speed video analysis, we present a scheme for the analysis of the rotational properties of multiple rod-shaped bacteria directly from video microscopy data. The bacterial body and flagella rotation frequency of Bacillus subtilis are determined by temporally resolved monitoring of the position of the bacterial body. In contrast to established methods, the video-based approach can be extended to the simultaneous analysis of several bacteria within the field of view. Monitoring multiple bacteria simultaneously will allow resolving the role of hydrodynamic interactions of multiple flagella motors on their mutual dynamics.

Citation Download Citation

Lena Dewenter, Christina Alpmann, Mike Woerdemann, and Cornelia Denz "Video-based analysis of the rotational behaviour of rod-shaped, self-propelled bacteria in holographic optical tweezers", Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 84270N (8 May 2012); https://doi.org/10.1117/12.922449

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