Patterned illumination for analysing neuronal function in 3D

Mary Ann Go; Christian Stricker; Steve Redman; Hans Bachor; Vincent Ricardo Daria

doi:10.1117/12.923421

8 May 2012 Patterned illumination for analysing neuronal function in 3D

Mary Ann Go, Christian Stricker, Steve Redman, Hans Bachor, Vincent Ricardo Daria

Proceedings Volume 8427, Biophotonics: Photonic Solutions for Better Health Care III; 842703 (2012) https://doi.org/10.1117/12.923421
Event: SPIE Photonics Europe, 2012, Brussels, Belgium

Abstract

We use patterned 3D multi-spot illumination to perform neuronal multi-site stimulation in rat brain tissue. Using a spatial light modulator, we holograpically project 3D light fields for multi-site two-photon photolysis of caged neurotransmitters to generate synaptic inputs to a neuron. Controlled photostimulation of multiple synapses from various locations in the dendritic tree provides a way to analyze how neurons integrate multiple inputs. Our holographic projection setup is incorporated into a two-photon 3D imaging microscope for visualization and for accurate positioning of specific uncaging sites along the neuron's dendritic tree. We show two-photon images and the neuron's response to holographic photostimulation of synapses along dendrites.

Citation Download Citation

Mary Ann Go, Christian Stricker, Steve Redman, Hans Bachor, and Vincent Ricardo Daria "Patterned illumination for analysing neuronal function in 3D", Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 842703 (8 May 2012); https://doi.org/10.1117/12.923421

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