Use of Raman microspectroscopy to score the malignancy of breast cancer cells

C. Nieva; M. Marro; R. Sanz-Pamplona; N. Santana-Codina; S. Rao; D. Petrov; A. Sierra

doi:10.1117/12.921356

8 May 2012 Use of Raman microspectroscopy to score the malignancy of breast cancer cells

C. Nieva, M. Marro, R. Sanz-Pamplona, N. Santana-Codina, S. Rao, D. Petrov, A. Sierra

Proceedings Volume 8427, Biophotonics: Photonic Solutions for Better Health Care III; 842713 (2012) https://doi.org/10.1117/12.921356
Event: SPIE Photonics Europe, 2012, Brussels, Belgium

Abstract

It has been described that benign MCF10A breast cancer cell line suffers phenotypic changes toward malignancy when are cultured in sparse conditions. Using Raman spectroscopy with an InVia Raman microscope (Renishaw) with a backscattered configuration, we have studied the metabolic changes of confluent and sparse MCF10A cell cultures. We used Principal Component Analysis and Partial Least Squares Discriminant Analyses to assess the different profiling of the metabolic composition of breast cancer cells. The results indicated that Raman spectroscopy together with multivariate analysis is a useful technique to distinguish metabolic changes in malignant transformation. The identification of new metabolites, implementing the catalogue on the characterization of the different phenotypes associated to cell malignancy using Raman spectroscopy is under study.

Citation Download Citation

C. Nieva, M. Marro, R. Sanz-Pamplona, N. Santana-Codina, S. Rao, D. Petrov, and A. Sierra "Use of Raman microspectroscopy to score the malignancy of breast cancer cells", Proc. SPIE 8427, Biophotonics: Photonic Solutions for Better Health Care III, 842713 (8 May 2012); https://doi.org/10.1117/12.921356

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KEYWORDS

Raman spectroscopy

Breast cancer

Principal component analysis

Statistical analysis

Photons

Remote sensing

Biomedical optics

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