Fluorescent Microscopy Enhancement Using Imaging

Morgan P. Conrad; Diether J. Reck tenwald; Bryan S. Woodhouse

doi:10.1117/12.975413

12 June 1986 Fluorescent Microscopy Enhancement Using Imaging

Morgan P. Conrad, Diether J. Reck tenwald, Bryan S. Woodhouse

Proceedings Volume 0626, Application of Optical Instrumentation in Medicine XIV and Picture Archiving and Communication Systems; (1986) https://doi.org/10.1117/12.975413
Event: Application of Optical Instrumentation in Medicine XIV and Picture Archiving and Communication Systems (PACS IV) for Medical Applications, 1986, Newport Beach, CA, United States

Abstract

To enhance our capabilities for observing fluorescent stains in biological systems, we are developing a low cost imaging system based around an IBM AT microcomputer and a commercial image capture board compatible with a standard RS-170 format video camera. The image is digitized in real time with 256 grey levels, while being displayed and also stored in memory. The software allows for interactive processing of the data, such as histogram equalization or pseudocolor enhancement of the display. The entire image, or a quadrant thereof, can be averaged over time to improve the signal to noise ratio. Images may be stored to disk for later use or comparison. The camera may be selected for better response in the UV or near IR. Combined with signal averaging, this increases the sensitivity relative to that of the human eye, while still allowing for the fluorescence distribution on either the surface or internal cytoskeletal structure to be observed.

Citation Download Citation

Morgan P. Conrad, Diether J. Reck tenwald, and Bryan S. Woodhouse "Fluorescent Microscopy Enhancement Using Imaging", Proc. SPIE 0626, Application of Optical Instrumentation in Medicine XIV and Picture Archiving and Communication Systems, (12 June 1986); https://doi.org/10.1117/12.975413

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