Ultrafast Laser Scanner Microscope: Review Of Design And Performance Testing

D Vukobratovich; P H Bartels; D Hillman; R Kingston; A Landesman; M Sabb; R L Shoemaker; R Shack

doi:10.1117/12.944330

9 June 1988 Ultrafast Laser Scanner Microscope: Review Of Design And Performance Testing

D Vukobratovich, P H Bartels, D Hillman, R Kingston, A Landesman, M Sabb, R L Shoemaker, R Shack

Proceedings Volume 0892, Simulation and Modeling of Optical Systems; (1988) https://doi.org/10.1117/12.944330
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

The ultrafast laser scanner microscope at the Optical Sciences Center, University of Arizona, was designed as a prototype for a new class of optical microscopes to serve directly as an optical data-acquisition peripheral for a computer. The design called for the ability to digitally record large specimen areas at high spatial resolution within a short period of time. These design parameters imply very high data rates and very large data volumes. Optical systems with the required point-spread function, such as conventional high-aperture microscope objectives, typically offer a field-of-view diameter of approximately 250 jam. Because this field is small, areas of frame overlap must be rescanned. Consequently, in the design of the laser-scanner objective, a very wide field of view was specified. The laser scanner design work began in 1981, and the instrument was completed in 1986. It has since been transferred to the Department of Pathology, where it is being used to digitally record histopathologic images. The instrument design implements a number of novel features. The following is a critical review of design and instrument performance features, particularly the optomechanical aspects of the instrument. Previous papers cover the design evolution of the ultrafast laser scanner microscope.^1-4

Citation Download Citation

D Vukobratovich, P H Bartels, D Hillman, R Kingston, A Landesman, M Sabb, R L Shoemaker, and R Shack "Ultrafast Laser Scanner Microscope: Review Of Design And Performance Testing", Proc. SPIE 0892, Simulation and Modeling of Optical Systems, (9 June 1988); https://doi.org/10.1117/12.944330

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available