Review Of Optical Methods In Immunosensing

Janusz W. Sadowski

doi:10.1117/12.947617

16 January 1989 Review Of Optical Methods In Immunosensing

Janusz W. Sadowski

Proceedings Volume 0954, Optical Testing and Metrology II; (1989) https://doi.org/10.1117/12.947617
Event: SPIE International Symposium on Optical Engineering and Industrial Sensing for Advance Manufacturing Technologies, 1988, Dearborn, MI, United States

Abstract

Currently within decentralised clinical laboratories there is a need for simple and rapid techniques to detect antigens and antibodies of diagnostic significance. The interaction between antigen and antibody molecules may be extremely specific as a consequence of binding geometries and is a principal phenomenon of antibody-based chemical sensors, i.e. immunosensors. Direct optical monitoring of this interaction is an attractive alternative in the development of immunosensors, because optical sensors can be very sensitive, small in size, safe, and cheap. This paper reviews immunosensors based on optical sensing techniques including such methods like light reflectance (the Brewster angle reflectometry, surface plasmon resonance), and evanescent wave fluorescence. The basic principles and the current state-of-the art are discussed with emphasis on sensitivity, measurement range, shortcomings and problems to be solved. Some comments on miniaturisation and future developments will also be given.

Citation Download Citation

Janusz W. Sadowski "Review Of Optical Methods In Immunosensing", Proc. SPIE 0954, Optical Testing and Metrology II, (16 January 1989); https://doi.org/10.1117/12.947617

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