Nanocapillaries and optical tweezers for studies on DNA in confinement

Oliver Otto; Lorenz J. Steinbock; Ulrich F. Keyser

doi:10.1117/12.894703

9 September 2011 Nanocapillaries and optical tweezers for studies on DNA in confinement

Oliver Otto, Lorenz J. Steinbock, Ulrich F. Keyser

Proceedings Volume 8097, Optical Trapping and Optical Micromanipulation VIII; 80970B (2011) https://doi.org/10.1117/12.894703
Event: SPIE NanoScience + Engineering, 2011, San Diego, California, United States

Abstract

We present measurements on single and multiple DNA molecules inside a nanocapillary. Nanocapillaries are single molecule sensors with similar properties as standard solid-state nanopores made from silicon nitride. For stalling of DNA in the nanocapillaries, we apply optical fiber illumination in combination with video detection for real-time tracking of optically trapped colloids on a microsecond time scale. Using a cross-correlation based algorithm after image acquisition from a CMOS camera we are able to measure the position of a colloid at rates up to 40,000 frames per second over hours. A full understanding of electrokinetic transport of polyelectrolytes in strongly confined environments is still elusive. In order to shed new light on this process we perform electrophoretic force and ionic-current measurements on single and multiple DNA molecules inside nanocapillaries attached to an optically trapped colloid. The hydrodynamic interaction between single DNA molecules was investigated by capturing multiple strands inside the tip of a capillary. We find that the capture force depends linearly on the number of DNA molecules.

Citation Download Citation

Oliver Otto, Lorenz J. Steinbock, and Ulrich F. Keyser "Nanocapillaries and optical tweezers for studies on DNA in confinement", Proc. SPIE 8097, Optical Trapping and Optical Micromanipulation VIII, 80970B (9 September 2011); https://doi.org/10.1117/12.894703

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KEYWORDS

Optical tweezers

Molecules

Capillaries

Microfluidics

Calibration

Light sources

Optical fibers

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