Characterization of microorganisms using Raman tweezers

Ota Samek; Zdenek Pilát; Alexandr Jonás; Pavel Zemánek; Mojmir Sery; Jan Jezek; Silvie Bernatová; Ladislav Nedbal; Martin Trtílek

doi:10.1117/12.893420

9 September 2011 Characterization of microorganisms using Raman tweezers

Ota Samek, Zdenek Pilát, Alexandr Jonás, Pavel Zemánek, Mojmir Sery, Jan Jezek, Silvie Bernatová, Ladislav Nedbal, Martin Trtílek

Author Affiliations +

Proceedings Volume 8097, Optical Trapping and Optical Micromanipulation VIII; 80970F (2011) https://doi.org/10.1117/12.893420
Event: SPIE NanoScience + Engineering, 2011, San Diego, California, United States

Abstract

The ability to identify and characterize microorganisms (algae, bacteria, eukaryotic cells) from minute sample volumes in a rapid and reliable way is the crucial first step in their classification and characterization. In the light of this challenge related to microorganisms exploitation Raman spectroscopy can be used as a powerful tool for chemical analysis. Raman spectroscopy can elucidate fundamental questions about the metabolic processes and intercellular variability on a single cell level. Moreover, Raman spectroscopy can be combined with optical tweezers and with microfluidic chips to measure nutrient dynamics and metabolism in vivo, in real-time, and label free. We demonstrate the feasibility to employ Raman spectroscopy-based sensor to sort microorganisms (bacteria, algae) according to the Raman spectra. It is now quite feasible to sort algal cells according to the degree of unsaturation (iodine value) in lipid storage bodies.

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Ota Samek, Zdenek Pilát, Alexandr Jonás, Pavel Zemánek, Mojmir Sery, Jan Jezek, Silvie Bernatová, Ladislav Nedbal, and Martin Trtílek "Characterization of microorganisms using Raman tweezers", Proc. SPIE 8097, Optical Trapping and Optical Micromanipulation VIII, 80970F (9 September 2011); https://doi.org/10.1117/12.893420

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