Viability studies of optically trapped T-cells

Niall McAlinden; David G. Glass; Owain Millington; Amanda J. Wright

doi:10.1117/12.895232

9 September 2011 Viability studies of optically trapped T-cells

Niall McAlinden, David G. Glass, Owain Millington, Amanda J. Wright

Proceedings Volume 8097, Optical Trapping and Optical Micromanipulation VIII; 80970J (2011) https://doi.org/10.1117/12.895232
Event: SPIE NanoScience + Engineering, 2011, San Diego, California, United States

Abstract

We present a viability study of optically trapped live T cell hybridomas. T cells form an important part of the adaptive immune response system which is responsible for fighting particular pathogens or diseases. The cells of interest were directly trapped by a laser operating at a wavelength of 1064 nm and their viability measured as a function of time. Cell death was monitored using an inverted fluorescent microscope to observe the uptake by the cell of the fluorescent dye propidium iodide. Studies were undertaken at various laser powers and beam profiles. There is a growing interest in optically trapping immune cells and this is the first study that investigates the viability of a T cell when trapped using a conventional optical trapping system. In such experiments it is crucial that the T cell remains viable and trapping the cell directly means that any artefacts due to a cell-bead interface are removed. Our motivation behind this experiment is to use optical tweezers to gain a greater understanding of the interaction forces between T cells and antigen presenting cells. Measuring these interactions has become important due to recent theories which indicate that the strength of this interaction may underlie the activation of the T-cell and subsequent immune response.

Citation Download Citation

Niall McAlinden, David G. Glass, Owain Millington, and Amanda J. Wright "Viability studies of optically trapped T-cells", Proc. SPIE 8097, Optical Trapping and Optical Micromanipulation VIII, 80970J (9 September 2011); https://doi.org/10.1117/12.895232

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