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Proceedings Article

In vivo time-resolved autofluorescence measurements on human skin

[+] Author Affiliations
Kamal M. Katika, Laurent Pilon, Jennifer Blackwell, Halil Berberoglu

Univ. of California, Los Angeles

Katrina Dipple

David Geffen School of Medicine, Univ. of California, Los Angeles

Seymour Levin

Wadsworth Veterans Administration Hospital

Proc. SPIE 6078, Photonic Therapeutics and Diagnostics II, 60780L (February 22, 2006); doi:10.1117/12.647149
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From Conference Volume 6078

  • Photonic Therapeutics and Diagnostics II
  • Nikiforos Kollias; Haishan Zeng; Bernard Choi; Reza S. Malek; Brian J. Wong; Justus F. R. Ilgner; Eugene A. Trowers; Werner T. de Riese; Henry Hirschberg; Steen J. Madsen; Michael D. Lucroy; Lloyd P. Tate; Kenton W. Gregory; Guillermo J. Tearney
  • San Jose, CA | January 21, 2006

abstract

In this paper we present preliminary results obtained from fluorescence lifetime measurements on human skin using time-correlated single photon counting (TCSPC) techniques. Human skin was exposed to light from a pulsed LED of 700 ps pulse width at a wavelength of 375 nm and fluorescence decays were recorded at four different emission wavelengths (442, 460, 478 and 496 nm) using a photomultiplier tube (PMT) coupled to a monochromator. Measurements were carried out on the left and right palms of subjects recruited for the study after obtaining consent using a UCLA IRB approved consent form. The subjects recruited consisted of 18 males and 17 females with different skin complexions and ages ranging from 10 to 70 years. In addition, a set of experiments were also performed on various locations including the palm, the arm and the cheek of a Caucasian subject. The fluorescence decays thus obtained were fit to a three-exponential decay model in all cases and were approximately 0.4, 2.7 and 9.4 ns, respectively. The variations in these lifetimes with location, gender, skin complexion and age are studied. It is speculated that the shorter lifetimes correspond to free and bound NADH while the longer lifetime is due to AGE crosslinks.

© (2006) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Kamal M. Katika ; Laurent Pilon ; Katrina Dipple ; Seymour Levin ; Jennifer Blackwell, et al.
"In vivo time-resolved autofluorescence measurements on human skin", Proc. SPIE 6078, Photonic Therapeutics and Diagnostics II, 60780L (February 22, 2006); doi:10.1117/12.647149; http://dx.doi.org/10.1117/12.647149


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