Flying spot en face OCT for monitoring cell distribution in collagen-based constructs

Konstantinos Kosmidis; Christopher D. Russell; Richard A. Black; George Dobre; Adrian Gh. Podoleanu

doi:10.1117/12.648886

20 February 2006 Flying spot en face OCT for monitoring cell distribution in collagen-based constructs

Konstantinos Kosmidis, Christopher D. Russell, Richard A. Black, George Dobre, Adrian Gh. Podoleanu

Proceedings Volume 6079, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine X; 60790Q (2006) https://doi.org/10.1117/12.648886
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.

Citation Download Citation

Konstantinos Kosmidis, Christopher D. Russell, Richard A. Black, George Dobre, and Adrian Gh. Podoleanu "Flying spot en face OCT for monitoring cell distribution in collagen-based constructs", Proc. SPIE 6079, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine X, 60790Q (20 February 2006); https://doi.org/10.1117/12.648886

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available