Development of a noninvasive multifunctional measurement method using nanosecond pulsed laser for evaluation of regenerative medicine for articular cartilage

Miya Ishihara; Masato Sato M.D.; Nagatoshi Kaneshiro M.D.; Genya Mitani M.D.; Shunichi Sato; Masayuki Ishihara; Joji Mochida M.D.; Makoto Kikuchi

doi:10.1117/12.645888

1 March 2006 Development of a noninvasive multifunctional measurement method using nanosecond pulsed laser for evaluation of regenerative medicine for articular cartilage

Miya Ishihara, Masato Sato M.D., Nagatoshi Kaneshiro M.D., Genya Mitani M.D., Shunichi Sato, Masayuki Ishihara, Joji Mochida M.D., Makoto Kikuchi

Author Affiliations +

Proceedings Volume 6084, Optical Interactions with Tissue and Cells XVII; 60840V (2006) https://doi.org/10.1117/12.645888
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

We demonstrated the capability of a photoacoustic method for viscoelastic measurement. The measurement method has already proved to be useful for evaluation of regenerative medicine of articular cartilage. However, characterization of the extracellular matrix as well as determination of the viscoelastic property should be carried out for evaluation of regenerative medicine because the extracellular matrix plays an important role. We therefore developed a method for characterization of the extracellular matrix that can be performed simultaneously with the photoacoustic measurement. Since collagen molecules, which are the major contents of the cartilage extracellular matrix, are well known as endogenous fluorescent molecules, it is possible that fluorescence measurement will enable characterization of the extracellular matrix. Third harmonic Q-switched Nd:YAG laser pulses were used as an excitation light source. The time-resolved fluorescence spectroscopy was obtained by using a photonic multi-channel analyzer. Tissue-engineered cartilages cultured under different conditions for various periods were used as samples. Different culture conditions resulted in different extracellular matrix formations. There were significant differences in the measured fluorescent parameters among the culture conditions of cartilage because chondrocytes produce a specific extracellular matrix depending on its culture condition. The specific extracellular matrix contained a specific type of collagen such as collagen type I or type II, which each have specific fluorescent features. Thus, the fluorescent parameters enabled characterization of synthesis of cartilage-associated extracellular matrix. Therefore, the combination of fluorescence and photoacoustic measurement is expected to become a useful evaluation method in regenerative medicine.

Citation Download Citation

Miya Ishihara, Masato Sato M.D., Nagatoshi Kaneshiro M.D., Genya Mitani M.D., Shunichi Sato, Masayuki Ishihara, Joji Mochida M.D., and Makoto Kikuchi "Development of a noninvasive multifunctional measurement method using nanosecond pulsed laser for evaluation of regenerative medicine for articular cartilage", Proc. SPIE 6084, Optical Interactions with Tissue and Cells XVII, 60840V (1 March 2006); https://doi.org/10.1117/12.645888

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available