Monitoring activity of living cells marked with colloidal semiconductor quantum dots

Patricia M. A. Farias; Beate S. Santos; Frederico D. Menezes; Aluizio G. Brasil, Jr.; Liliane B. Lima; Diego C. N. Silva; Alice A. S. Vieira; Ricardo Ferreira; Adriana Fontes; Diogo Almeida; Luiz C. Barbosa; Carlos L. Cesar

doi:10.1117/12.646817

21 February 2006 Monitoring activity of living cells marked with colloidal semiconductor quantum dots

Patricia M. A. Farias, Beate S. Santos, Frederico D. Menezes, Aluizio G. Brasil, Jr., Liliane B. Lima, Diego C. N. Silva, Alice A. S. Vieira, Ricardo Ferreira, Adriana Fontes, Diogo Almeida, Luiz C. Barbosa, Carlos L. Cesar

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Proceedings Volume 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV; 60880G (2006) https://doi.org/10.1117/12.646817
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Fluorescent semiconductor nanocrystals in quantum confinement regime (quantum dots) present several well known features which make them very useful tools for biological labeling purposes. Low photo-bleaching rates, high chemical stability, active surface allowing conjugation to living cells, explains the success of this labeling procedure over the commonly used fluorescent dyes. In this paper we report the results obtained with high fluorescent core-shell CdTe-CdS (diameter = 3-7 nm) colloidal nanocrystals synthesized in aqueous medium and conjugated to glucose molecules, incubated with living yeast cells, in order to investigate their glucose up-take activity.

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Patricia M. A. Farias, Beate S. Santos, Frederico D. Menezes, Aluizio G. Brasil, Jr., Liliane B. Lima, Diego C. N. Silva, Alice A. S. Vieira, Ricardo Ferreira, Adriana Fontes, Diogo Almeida, Luiz C. Barbosa, and Carlos L. Cesar "Monitoring activity of living cells marked with colloidal semiconductor quantum dots", Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 60880G (21 February 2006); https://doi.org/10.1117/12.646817

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KEYWORDS

Quantum dots

Glucose

Yeast

Luminescence

Nanocrystals

Semiconductors

Confocal microscopy

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