Investigation of 3D microscopy using intensity diffraction tomography

Mark A. Anastasio; Yin Huang; Greg Gbur; P. Scott Carney

doi:10.1117/12.648169

23 February 2006 Investigation of 3D microscopy using intensity diffraction tomography

Mark A. Anastasio, Yin Huang, Greg Gbur, P. Scott Carney

Proceedings Volume 6090, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII; 60900G (2006) https://doi.org/10.1117/12.648169
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Diffraction tomography (DT) is an established imaging technique for reconstructing the complex-valued refractive index distribution of a weakly scattering 3D sample. Due to experimental difficulties associated with the direct measurement of the phase of an optical wavefield, the effectiveness of DT for optical imaging applications has been limited. A theory of intensity diffraction tomography (I-DT) has been proposed to circumvent this phase retrieval problem. In this work, we review the features of I-DT reconstruction theory that are relevant to optical microscopy. Computer-simulation studies are conducted to investigate the performance of reconstruction algorithms for a proposed I-DT microscope. The effects of data noise are assessed, and statistically optimal reconstruction strategies that employ multiple detector planes are proposed.

Citation Download Citation

Mark A. Anastasio, Yin Huang, Greg Gbur, and P. Scott Carney "Investigation of 3D microscopy using intensity diffraction tomography", Proc. SPIE 6090, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII, 60900G (23 February 2006); https://doi.org/10.1117/12.648169

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