Use of the second harmonic generation microscopy to evaluate chondrogenic differentiation of mesenchymal stem cells for cartilage repair

P. Bordeaux-Rego; M. O. Baratti; A. S. S. Duarte; T. B. Ribeiro; M. F. Andreoli-Risso; B. Vidal; J. B. Miranda; J. Adur; A. A. de Thomaz; V. B. Pelegati; F. F. Costa; H. F. Carvalho; C. L. Cesar; A. Luzo; S. T. Olalla Saad

doi:10.1117/12.909185

9 February 2012 Use of the second harmonic generation microscopy to evaluate chondrogenic differentiation of mesenchymal stem cells for cartilage repair

P. Bordeaux-Rego, M. O. Baratti, A. S. S. Duarte, T. B. Ribeiro, M. F. Andreoli-Risso, B. Vidal, J. B. Miranda, J. Adur, A. A. de Thomaz, V. B. Pelegati, F. F. Costa, H. F. Carvalho, C. L. Cesar, A. Luzo, S. T. Olalla Saad

Author Affiliations +

Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 82263N (2012) https://doi.org/10.1117/12.909185
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Articular cartilage injury remains one of the major concerns in orthopedic surgery. Mesenchymal stem cell (MSC) transplantation has been introduced to avoid some of the side effects and complications of current techniques.. With the aim to evaluate chondrogenic differentiation of mesenchymal stem cells, we used Second Harmonic Generation (SHG) microscopy to analyze the aggregation and orientation of collagen fibrils in the hyaline cartilage of rabbit knees. The experiment was performed using implants with type II collagen hydrogel (a biomaterial that mimics the microenvironment of the cartilage), one implant containing MSC and one other without MSC (control). After 10 weeks, the rabbit knees were dissected and fibril collagen distribution and spatial organization in the extracellular matrix of the lesions were verified by SHG. The result showed significant differences, whereas in histological sections of the cartilaginous lesions with MSC the collagen fibers are organized and regular; in the control sections the collagen fibers are more irregular, with absence of cells. A macroscopic analysis of the lesions confirmed this difference, showing a greater percentage of lesions filling in knees treated with MSC than in the knees used as controls. This study demonstrates that SHG microscopy will be an excellent tool to help in the evaluation of the effectiveness of MSC-based cell therapy for cartilage repair.

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P. Bordeaux-Rego, M. O. Baratti, A. S. S. Duarte, T. B. Ribeiro, M. F. Andreoli-Risso, B. Vidal, J. B. Miranda, J. Adur, A. A. de Thomaz, V. B. Pelegati, F. F. Costa, H. F. Carvalho, C. L. Cesar, A. Luzo, and S. T. Olalla Saad "Use of the second harmonic generation microscopy to evaluate chondrogenic differentiation of mesenchymal stem cells for cartilage repair", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82263N (9 February 2012); https://doi.org/10.1117/12.909185

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