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Proceedings Article

In vivo pump-probe microscopy of melanoma and pigmented lesions

[+] Author Affiliations
Jesse W. Wilson, Simone Degan, M. Angelica Selim, Jennifer Y. Zhang, Warren S. Warren

Duke Univ. (USA)

Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822602 (February 9, 2012); doi:10.1117/12.908821
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From Conference Volume 8226

  • Multiphoton Microscopy in the Biomedical Sciences XII
  • Karsten König
  • San Francisco, California, USA | January 21, 2012

abstract

A growing number of dermatologists and pathologists are concerned that the rapidly rising incidence of melanoma reflects not a true 'epidemic' but an increasing tendency to overdiagnose pigmented lesions. Addressing this problem requires both a better understanding of early-stage melanoma and new diagnostic criteria based on more than just cellular morphology and architecture. Here we present a method for in-vivo optical microscopy that utilizes pump-probe spectroscopy to image the distribution of the two forms of melanin in skin: eumelanin and pheomelanin. Images are acquired in a scanning microscope with a sensitive modulation transfer technique by analyzing back-scattered probe light with a lock-in amplifier. Early-stage melanoma is studied in a human skin xenografted mouse model. Individual melanocytes have been observed, in addition to pigmented keratinocytes. Combining the pump-probe images simultaneously with other noninvasive laser microscopy methods (confocal reflectance, multiphoton autofluorescence, and second harmonic generation) allows visualization of the skin architecture, framing the functional pump-probe image in the context of the surrounding tissue morphology. It is found that pump-probe images of melanin can be acquired with low peak intensities, enabling wide field-of-view pigmentation surveys. Finally, we investigate the diagnostic potential of the additional chemical information available from pump-probe microscopy.

© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Jesse W. Wilson ; Simone Degan ; M. Angelica Selim ; Jennifer Y. Zhang and Warren S. Warren
"In vivo pump-probe microscopy of melanoma and pigmented lesions", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822602 (February 9, 2012); doi:10.1117/12.908821; http://dx.doi.org/10.1117/12.908821


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