Facile synthesis of fluorescent gold nanoclusters and their application in cellular imaging

Li Shang; René M. Dörlich; Stefan Brandholt; Naghmeh Azadfar; Gerd Ulrich Nienhaus

doi:10.1117/12.906250

2 February 2012 Facile synthesis of fluorescent gold nanoclusters and their application in cellular imaging

Li Shang, René M. Dörlich, Stefan Brandholt, Naghmeh Azadfar, Gerd Ulrich Nienhaus

Proceedings Volume 8232, Colloidal Nanocrystals for Biomedical Applications VII; 82321J (2012) https://doi.org/10.1117/12.906250
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Fluorescent metal nanoclusters (NCs) have many attractive features, including ultrasmall size, good biocompatibility and large Stokes shift, thus making them promising alternatives as fluorescent markers for biological applications. We have developed a facile method of synthesizing water-soluble, near-infrared (NIR) luminescent AuNCs. By virtue of the long fluorescence lifetime of these AuNCs, we explored their application in cellular fluorescence lifetime imaging (FLIM). Furthermore, the interaction of AuNCs with biomolecules such as proteins has been investigated. Upon protein association, the fluorescence of AuNCs was found to be enhanced and their luminescence lifetime was prolonged. Our studies underline the importance of studying the behavior of AuNCs within the complex biological environment for their application as novel cellular imaging probes.

Citation Download Citation

Li Shang, René M. Dörlich, Stefan Brandholt, Naghmeh Azadfar, and Gerd Ulrich Nienhaus "Facile synthesis of fluorescent gold nanoclusters and their application in cellular imaging", Proc. SPIE 8232, Colloidal Nanocrystals for Biomedical Applications VII, 82321J (2 February 2012); https://doi.org/10.1117/12.906250

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