Femtosecond laser two-photon polymerization of three-dimensional scaffolds for tissue engineering and regenerative medicine applications

V. Aprile; S. M. Eaton; M. Laganà; G. Cerullo; M. T. Raimondi; R. Osellame

doi:10.1117/12.907847

15 February 2012 Femtosecond laser two-photon polymerization of three-dimensional scaffolds for tissue engineering and regenerative medicine applications

V. Aprile, S. M. Eaton, M. Laganà, G. Cerullo, M. T. Raimondi, R. Osellame

Author Affiliations +

Proceedings Volume 8247, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XII; 824708 (2012) https://doi.org/10.1117/12.907847
Event: SPIE LASE, 2012, San Francisco, California, United States

Abstract

Recent studies have shown that mechanical factors can direct stem cell fate in vitro, even in the absence of biochemical factors. Two-photon laser polymerization was applied here to fabricate ultra-precise 3D micro-scaffolds with different architectures and pore sizes able to structurally interact with cells at the single-cell scale. Our experiments have shown that randomly seeded mesenchymal stem cells systematically colonize the internal volumes of 3D scaffolds and proliferate, while showing a roundish morphology. Even if stem cell mechanobiology is a very complex field, this study shows how mechanical interactions studied in a 3D micro-architecture at a single cell scale may influence stem cells response.

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V. Aprile, S. M. Eaton, M. Laganà, G. Cerullo, M. T. Raimondi, and R. Osellame "Femtosecond laser two-photon polymerization of three-dimensional scaffolds for tissue engineering and regenerative medicine applications", Proc. SPIE 8247, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XII, 824708 (15 February 2012); https://doi.org/10.1117/12.907847

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