Optical computed-tomographic microscopy: new technology for three-dimensional imaging of cells

Ravil Chamgoulov; Pierre Lane; Calum MacAulay

doi:10.1117/12.696932

21 July 2006 Optical computed-tomographic microscopy: new technology for three-dimensional imaging of cells

Ravil Chamgoulov, Pierre Lane, Calum MacAulay

Proceedings Volume 6163, Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII; 616306 (2006) https://doi.org/10.1117/12.696932
Event: Saratov Fall Meeting 2005, 2005, Saratov, Russian Federation

Abstract

Our group at the BC Cancer Research Centre has developed an optical computed-tomography microscope for three-dimensional (3-D) imaging and quantitative analysis of absorption-stained biological samples. The device uses non-ionizing optical radiation for illumination and computed-tomography for 3-D image reconstruction. The optical system consists of a light source, two high-numerical aperture (NA) objective lenses, a sample stage, a CCD detector, and a spatial light modulator that is used for computer-controlled sample illumination. Projections of a specimen are measured at a set of appointed angles within the range of 0 less than or equal to angle less than or equal to 135, which is limited by the NA of the objective. The reconstruction algorithm developed to solve the limited-angle problem employs the reconstruction from a transform-based method as an initial guess for the following iterative reconstruction. 3-D microscopic images of quantitatively absorption-stained cells have been generated with the resolution on the order of 1 micron. Visualization of 3-D tissue structure and architectural and morphological features that can be extracted from the volume to provide pathologists with additional information for diagnostic purposes.

Citation Download Citation

Ravil Chamgoulov, Pierre Lane, and Calum MacAulay "Optical computed-tomographic microscopy: new technology for three-dimensional imaging of cells", Proc. SPIE 6163, Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII, 616306 (21 July 2006); https://doi.org/10.1117/12.696932

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