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Proceedings Article

Development of a compact bio-optofluidic cell sorter

[+] Author Affiliations
Andrew Banas, Darwin Palima, Finn Pedersen, Jesper Glückstad

Technical Univ. of Denmark (Denmark)

Proc. SPIE 8274, Complex Light and Optical Forces VI, 82740N (February 9, 2012); doi:10.1117/12.909908
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From Conference Volume 8274

  • Complex Light and Optical Forces VI
  • Enrique J. Galvez; David L. Andrews; Jesper Glückstad; Marat S. Soskin
  • San Francisco, California, USA | January 21, 2012

abstract

We develop an active cell sorter that utilizes machine vision for cell identification. Particles are identified based on visual features such as shape, size and color using image processing. The sorter shares features from our previously developed BioPhotonics Workstation. Hence, it benefits from the extended axial manipulation range provided by the low numerical aperture geometry. Detected particles are catapulted axially by several hundred microns, allowing them to be moved from one laminar flow region to another. As the sorting motion is transverse to the viewing plane, multiple particles can be catapulted at the same time, therefore enabling parallel sorting. The sorter is developed with a minimal footprint such that it can operate as a table top device, an advantage over flow cytometry or FACS systems.

© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Andrew Banas ; Darwin Palima ; Finn Pedersen and Jesper Glückstad
"Development of a compact bio-optofluidic cell sorter", Proc. SPIE 8274, Complex Light and Optical Forces VI, 82740N (February 9, 2012); doi:10.1117/12.909908; http://dx.doi.org/10.1117/12.909908


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