On-chip SERS analysis for single mimic pathogen detection using Raman-labeled nanoaggregate-embedded beads with a dielectrophoretic chip

Chen-Han Huang; Hsing-Ying Lin; I-Ting Kuo; Wen-Hsin Hsieh; Ping-Ji Huang; Tzyy-Schiuan Yang; Lai-Kwan Chau

doi:10.1117/12.914552

30 January 2012 On-chip SERS analysis for single mimic pathogen detection using Raman-labeled nanoaggregate-embedded beads with a dielectrophoretic chip

Chen-Han Huang, Hsing-Ying Lin, I-Ting Kuo, Wen-Hsin Hsieh, Ping-Ji Huang, Tzyy-Schiuan Yang, Lai-Kwan Chau

Author Affiliations +

Proceedings Volume 8351, Third Asia Pacific Optical Sensors Conference; 83512T (2012) https://doi.org/10.1117/12.914552
Event: Asia Pacific Optical Sensors Conference, 2012, Sydney, Australia

Abstract

The integration of Raman-labeled nanoaggregate-embedded beads (NAEBs) for high performance SERS analysis of single mimic pathogen on a self-designed dielectrophoretic chip is demonstrated. The Raman tags called NAEBs are silica-coated, dye-induced aggregates of a small number of gold nanoparticles (AuNPs). In this work, NAEBs consisting of a Raman dye tetramethyl-rhodamine-5-isothiosyanate (TRITC) are chemically functionalized with streptavidin to detect biotin-functionalized polystyrene (PS) microspheres which mimic as pathogens. The sample solution of completely mixed streptavidin-functionalized NAEBs and biotin-functionalized PS microspheres is pumped into the microfluidic channel of a dielectrophoretic chip. By giving an AC voltage on the embedded electrodes, a single mimic pathogen can be caught via the non-contact dielectrophoretic force and suspended at the central cross of four aluminum electrodes for subsequent Raman spectroscopic detection. The SERS signal of TRITC is used as a spectral signature of specific mimic pathogen recognition, otherwise only the background Raman signal of a PS microsphere is observed. A pathogen-specific biosensor based on the dielectrophoresis-Raman spectroscopy system is developed, and the proof-ofconcept is confirmed by the specific molecular interaction model of streptavidin with biotin. Therefore, the on-chip multiplex SERS analysis of pathogens can be anticipated by employing different dye-tagged NAEBs simultaneously in a sample solution. We believe this bioassay has the ability to screen and detect multiple pathogens with minimal sample processing and handling even a small number of pathogens is present.

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Chen-Han Huang, Hsing-Ying Lin, I-Ting Kuo, Wen-Hsin Hsieh, Ping-Ji Huang, Tzyy-Schiuan Yang, and Lai-Kwan Chau "On-chip SERS analysis for single mimic pathogen detection using Raman-labeled nanoaggregate-embedded beads with a dielectrophoretic chip", Proc. SPIE 8351, Third Asia Pacific Optical Sensors Conference, 83512T (30 January 2012); https://doi.org/10.1117/12.914552

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