Wave front engineering by means of diffractive optical elements for applications in microscopy

Dan Cojoc; Enrico Ferrari; Valeria Garbin; Stefano Cabrini; Alessandro Carpentiero; Mauro Prasciolu; Luca Businaro; Burchard Kaulich; Enzo Di Fabrizio

doi:10.1117/12.679941

14 June 2006 Wave front engineering by means of diffractive optical elements for applications in microscopy

Dan Cojoc, Enrico Ferrari, Valeria Garbin, Stefano Cabrini, Alessandro Carpentiero, Mauro Prasciolu, Luca Businaro, Burchard Kaulich, Enzo Di Fabrizio

Author Affiliations +

Proceedings Volume 6254, Seventh International Conference on Correlation Optics; 625416 (2006) https://doi.org/10.1117/12.679941
Event: Seventh International Conference on Correlation Optics, 2005, Chernivsti, Ukraine

Abstract

We present a unified view regarding the use of diffractive optical elements (DOEs) for microscopy applications a wide range of electromagnetic spectrum. The unified treatment is realized through the design and fabrication of DOE through which wave front beam shaping is obtained. In particular we show applications ranging from micromanipulation using optical tweezers to X-ray differential interference contrast (DIC) microscopy. We report some details on the design and physical implementation of diffractive elements that beside focusing perform also other optical functions: beam splitting, beam intensity and phase redistribution or mode conversion. Laser beam splitting is used for multiple trapping and independent manipulation of spherical micro beads and for direct trapping and manipulation of biological cells with non-spherical shapes. Another application is the Gauss to Laguerre-Gaussian mode conversion, which allows to trap and transfer orbital angular momentum of light to micro particles with high refractive index and to trap and manipulate low index particles. These experiments are performed in an inverted optical microscope coupled with an infrared laser beam and a spatial light modulator for DOEs implementation. High resolution optics, fabricated by means of e-beam lithography, are demonstrated to control the intensity and the phase of the sheared beams in X-ray DIC microscopy. DIC experiments with phase objects reveal a dramatic increase in image contrast compared to bright-field X-ray microscopy.

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Dan Cojoc, Enrico Ferrari, Valeria Garbin, Stefano Cabrini, Alessandro Carpentiero, Mauro Prasciolu, Luca Businaro, Burchard Kaulich, and Enzo Di Fabrizio "Wave front engineering by means of diffractive optical elements for applications in microscopy", Proc. SPIE 6254, Seventh International Conference on Correlation Optics, 625416 (14 June 2006); https://doi.org/10.1117/12.679941

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