Multiwavelength fluorescence lifetime imaging by TCSPC

Axel Bergmann; Wolfgang Becker

doi:10.1117/12.684743

25 October 2006 Multiwavelength fluorescence lifetime imaging by TCSPC

Axel Bergmann, Wolfgang Becker

Proceedings Volume 6372, Advanced Photon Counting Techniques; 637204 (2006) https://doi.org/10.1117/12.684743
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

We present an approach which monitors both time- and spectral information of the fluorescence in order to receive the full information content of the light emitted from a sample. Our instrumentation covers a combination of the TCSPC technique extended by a multi-wavelength detection scheme. Based on the spectral properties of the participating chromophores their relative contributions to the fluorescence within each wavelength channel can be derived. This information serves as an additional parameter for the decay curve analysis which allows us to identify emission patterns for both lifetime and wavelength. Potential applications of the multi-wavelength TCSPC technique are FRET experiments, new marker techniques based on environment-dependent lifetime, and the separation of fluorophores in autofluorescence images of tissue.

Citation Download Citation

Axel Bergmann and Wolfgang Becker "Multiwavelength fluorescence lifetime imaging by TCSPC", Proc. SPIE 6372, Advanced Photon Counting Techniques, 637204 (25 October 2006); https://doi.org/10.1117/12.684743

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