Diffuse optical fluorescence tomography using time-resolved data acquired in transmission

Frederic Leblond; Simon Fortier; Michael P. Friedlander

doi:10.1117/12.700841

20 February 2007 Diffuse optical fluorescence tomography using time-resolved data acquired in transmission

Frederic Leblond, Simon Fortier, Michael P. Friedlander

Proceedings Volume 6431, Multimodal Biomedical Imaging II; 643106 (2007) https://doi.org/10.1117/12.700841
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We present an algorithm using data acquired with a time-resolved system with the goal of reconstructing sources of fluorescence emanating from the deep interior of highly scattering biological tissues. A novelty in our tomography algorithm is the integration of a light transport model adapted to rodent geometries. For small volumes, our analysis suggest that neglecting the index of refraction mismatch between diffusive and non-diffusive regions, as well as the curved nature of the boundary, can have a profound impact on fluorescent images and spectroscopic applications relying on diffusion curve fitting. Moreover, we introduce a new least-squares solver with bound constraints adapted for optical problems where a physical non-negative constraint can be imposed. Finally, we find that maximizing the time-related information content of the data in the reconstruction process significantly enhances the quality of fluorescence images. Preliminary noise propagation and detector placement optimization analysis are also presented.

Citation Download Citation

Frederic Leblond, Simon Fortier, and Michael P. Friedlander "Diffuse optical fluorescence tomography using time-resolved data acquired in transmission", Proc. SPIE 6431, Multimodal Biomedical Imaging II, 643106 (20 February 2007); https://doi.org/10.1117/12.700841

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