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Proceedings Article

Confocal time-resolved fluorescence anisotropy imaging

[+] Author Affiliations
Arjen N. Bader, Erik G. Hofman, Paul van Bergen en Henegouwen, Hans C. Gerritsen

Utrecht Univ. (Netherlands)

Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410C (February 19, 2007); doi:10.1117/12.702031
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From Conference Volume 6441

  • Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
  • Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau
  • San Jose, CA | January 20, 2007

abstract

A confocal time-resolved fluorescence anisotropy imaging set-up is presented. It combines a confocal laser scanning microscope equipped with a pulsed laser and two time gated detection systems with 4 gates each (LiMo, originally developed for FLIM). The anisotropy decays obtained with the time gating system yield results that compare well with the high time-resolution (non-imaging) decays recorded using Time Correlated Single Photon Counting. Time resolved anisotropy imaging experiments on cells expressing GPI-GFP were carried out. Clear distinction could be made between the anisotropy in the plasma membrane and in the interior of the cell.

© (2007) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Arjen N. Bader ; Erik G. Hofman ; Paul van Bergen en Henegouwen and Hans C. Gerritsen
"Confocal time-resolved fluorescence anisotropy imaging", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410C (February 19, 2007); doi:10.1117/12.702031; http://dx.doi.org/10.1117/12.702031


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