Confocal time-resolved fluorescence anisotropy imaging

Arjen N. Bader; Erik G. Hofman; Paul van Bergen en Henegouwen; Hans C. Gerritsen

doi:10.1117/12.702031

19 February 2007 Confocal time-resolved fluorescence anisotropy imaging

Arjen N. Bader, Erik G. Hofman, Paul van Bergen en Henegouwen, Hans C. Gerritsen

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 64410C (2007) https://doi.org/10.1117/12.702031
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

A confocal time-resolved fluorescence anisotropy imaging set-up is presented. It combines a confocal laser scanning microscope equipped with a pulsed laser and two time gated detection systems with 4 gates each (LiMo, originally developed for FLIM). The anisotropy decays obtained with the time gating system yield results that compare well with the high time-resolution (non-imaging) decays recorded using Time Correlated Single Photon Counting. Time resolved anisotropy imaging experiments on cells expressing GPI-GFP were carried out. Clear distinction could be made between the anisotropy in the plasma membrane and in the interior of the cell.

Citation Download Citation

Arjen N. Bader, Erik G. Hofman, Paul van Bergen en Henegouwen, and Hans C. Gerritsen "Confocal time-resolved fluorescence anisotropy imaging", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410C (19 February 2007); https://doi.org/10.1117/12.702031

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