TIRET microscopy: monitoring protein (amyloid precursor protein and beta-secretase) interaction on the surface of living cells

Christine von Arnim; Michael Wagner; Petra Weber; Herbert Schneckenburger

doi:10.1117/12.699856

19 February 2007 TIRET microscopy: monitoring protein (amyloid precursor protein and beta-secretase) interaction on the surface of living cells

Christine von Arnim, Michael Wagner, Petra Weber, Herbert Schneckenburger

Author Affiliations +

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 64410D (2007) https://doi.org/10.1117/12.699856
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Total internal reflection fluorescence microscopy (TIRFM) and non-radiative energy transfer (FRET) measurements have been combined in order to examine co-localization of the amyloid precursor protein (APP) and the ?-site APPcleaving enzyme (BACE) in human glioblastoma cells. So far, these proteins have been co-localized within whole cells (depending on the intracellular amount of cholesterol) and in some cases also within their plasma membranes. This supports the present hypothesis of localization within lipid domains on the cell surface and co-internalization via endocytosis.

Citation Download Citation

Christine von Arnim, Michael Wagner, Petra Weber, and Herbert Schneckenburger "TIRET microscopy: monitoring protein (amyloid precursor protein and beta-secretase) interaction on the surface of living cells", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410D (19 February 2007); https://doi.org/10.1117/12.699856

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