Single-SLM 3D interactive micromanipulation based on the generalized phase contrast (GPC) approach

Peter John Rodrigo; Ivan R. Perch-Nielsen; Carlo Amadeo Alonzo; Jesper Glückstad

doi:10.1117/12.700030

19 February 2007 Single-SLM 3D interactive micromanipulation based on the generalized phase contrast (GPC) approach

Peter John Rodrigo, Ivan R. Perch-Nielsen, Carlo Amadeo Alonzo, Jesper Glückstad

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 644110 (2007) https://doi.org/10.1117/12.700030
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Using a single phase-only spatial light modulator (SLM), we present a compact GPC-based optical trapping system for interactively manipulating microscopic particles in three dimensions (3D) and in real-time. We employ only one GPC 4f setup, which transforms 2D phase into intensity patterns, and utilize the SLM to form two phase-encoding regions defined by two equally sized apertures - one centered at x = x₀ and the other at x = -x₀ (with the optical axis centered at x = 0). Reconfigurable intensity patterns associated with the two independently addressable SLM-apertures are relayed to the sample volume to form a dynamic array of counterpropagating-beam traps. We discuss the experimental demonstrations showing 3D trapping of microparticles using the presented optical setup.

Citation Download Citation

Peter John Rodrigo, Ivan R. Perch-Nielsen, Carlo Amadeo Alonzo, and Jesper Glückstad "Single-SLM 3D interactive micromanipulation based on the generalized phase contrast (GPC) approach", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 644110 (19 February 2007); https://doi.org/10.1117/12.700030

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