Epi-detected Coherent anti-Stokes Raman Scattering Imaging of Deep Tissues in vivo

Terry B. Huff; Ji-Xin Cheng

doi:10.1117/12.699619

10 February 2007 Epi-detected coherent anti-Stokes Raman scattering imaging of deep tissues in vivo

Terry B. Huff, Ji-Xin Cheng

Proceedings Volume 6442, Multiphoton Microscopy in the Biomedical Sciences VII; 64420E (2007) https://doi.org/10.1117/12.699619
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We report in vivo molecular imaging of mouse sciatic nerve by epi-detected coherent anti-Stokes Raman Scattering (E-CARS) microscopy with vibrational selectivity, high signal-to-background ratio, 3D spatial resolution, and real-time imaging capability. The large CARS signal from the CH₂ stretch vibration allows highly sensitive and selective imaging of the myelin membrane which possesses a high lipid to protein ratio. The underlying contrast mechanism of in vivo CARS is explored by 3D imaging of fat cells that surround the nerve as well as dermal adipocytes in the mouse ear. Simultaneous E-CARS imaging of myelinated axons and second harmonic generation imaging of the surrounding collagen fibers were performed in vivo without any labeling. Finally, we show that CARS microscopy is able to distinguish between healthy myelin and disintegrated myelin induced by lysolecithin based on decrease in E-CARS intensity as well as loss of dependence on excitation polarization. Our system provides a multimodality in vivo imaging tool for studying neurodegenerative disorders.

Citation Download Citation

Terry B. Huff and Ji-Xin Cheng "Epi-detected coherent anti-Stokes Raman scattering imaging of deep tissues in vivo", Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64420E (10 February 2007); https://doi.org/10.1117/12.699619

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