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Proceedings Article

Calibration of a phase-shifting DIC microscope for quantitative phase imaging

[+] Author Affiliations
Sharon V. King, Ariel R. Libertun

Univ. of Colorado, Boulder

Chrysanthe Preza

Univ. of Memphis

Carol J. Cogswell

Univ. of Colorado, Boulder and CDM Optics, Inc.

Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 64430M (February 14, 2007); doi:10.1117/12.725061
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From Conference Volume 6443

  • Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV
  • Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson
  • San Jose, CA | January 20, 2007

abstract

Phase-shifting differential interference contrast (DIC) provides images in which the intensity of DIC is transformed into values linearly proportional to differential phase delay. Linear regression analysis of the Fourier space, spiral phase, integration technique shows these values can be integrated and calibrated to provide accurate phase measurements of objects embedded in optically transparent media regardless of symmetry or absorption properties. This approach has the potential to overcome the limitations of profilometery, which cannot access embedded objects, and extend the capabilities of the traditional DIC microscope, which images embedded phase objects, but does not provide quantitative information.

© (2007) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Sharon V. King ; Ariel R. Libertun ; Chrysanthe Preza and Carol J. Cogswell
"Calibration of a phase-shifting DIC microscope for quantitative phase imaging", Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 64430M (February 14, 2007); doi:10.1117/12.725061; http://dx.doi.org/10.1117/12.725061


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