Comparison of three-dimensional transfer function analysis of alternative phase imaging methods

Shan Shan Kou; Colin J. R. Sheppard

doi:10.1117/12.698995

14 February 2007 Comparison of three dimensional transfer function analysis of alternative phase imaging methods

Shan Shan Kou, Colin J. R. Sheppard

Proceedings Volume 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV; 64430Q (2007) https://doi.org/10.1117/12.698995
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Phase imaging is an invaluable tool for observation of biological sample, especially for living cells, where staining might not be appropriate, or for materials that do not absorb stain. Imaging of phase distributions with high spatial resolution can be used to derive the actual thickness and refractive index variations in the specimen. The detection of very small phase variations enables the detailed structure in the specimen to be revealed. As a result, the development and utilization of various phase imaging modalities have been important aspects of microscopy research. Differential Interference Contrast (DIC) and Quantitative Phase Microcopy (QPM) are based on partially coherent light, thus enabling high-resolution imaging. However, the low coherence requirement prevents the acquisition of quantitative phase data directly. On the other hand, Digital Holography Microscopy (DHM) is able to yield quantitative phase information but is compromised on resolution and cannot give full three dimensional (3D) reconstructions. In this paper, we present the 3D theoretical formalism of the above mentioned phase imaging methods with the focus on DHM. A comparative analysis here through visualization of 3D optical transfer functions gives an insight into the behaviors of these phase imaging methods.

Citation Download Citation

Shan Shan Kou and Colin J. R. Sheppard "Comparison of three dimensional transfer function analysis of alternative phase imaging methods", Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 64430Q (14 February 2007); https://doi.org/10.1117/12.698995

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available