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Proceedings Article

Observation of cell dynamics by laser scanning Raman microscope

[+] Author Affiliations
Keisaku Hamada, Katsumasa Fujita

Osaka Univ. (Japan)

Minoru Kobayashi

Nanophoton Corp. (Japan)

Satoshi Kawata

Osaka Univ. (Japan) and RIKEN (Japan)

Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 64430Z (February 14, 2007); doi:10.1117/12.700729
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From Conference Volume 6443

  • Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV
  • Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson
  • San Jose, CA | January 20, 2007

abstract

We developed a Raman microscope using a slit-scanning technique for observation of biological samples. A sample was illuminated by a line-shaped laser light, and Raman spectra were measured at different points in the line simultaneously by a spectrometer equipped with a 2D detector. The parallel detection of the Raman spectra boosts the image acquisition rate, which enable us to observe a living biological sample with high temporal and spatial resolution. We also applied a noise reduction technique using singular value decomposition. We recorded motion of intracellular components of living HeLa cells as sequential Raman images in a spectral region between 600 - 3000 cm-1 with the temporal resolution of 3 minutes.

© (2007) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Keisaku Hamada ; Katsumasa Fujita ; Minoru Kobayashi and Satoshi Kawata
"Observation of cell dynamics by laser scanning Raman microscope", Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 64430Z (February 14, 2007); doi:10.1117/12.700729; http://dx.doi.org/10.1117/12.700729


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