Programmable diffractive optics for laser scanning confocal microscopy

B. R. Boruah; M. A. A. Neil

doi:10.1117/12.700611

14 February 2007 Programmable diffractive optics for laser scanning confocal microscopy

B. R. Boruah, M. A. A. Neil

Proceedings Volume 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV; 644310 (2007) https://doi.org/10.1117/12.700611
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

In this work, we describe the design and implementation of a laser scanning confocal microscope with active beam forming optics. We demonstrate dynamic control over intensity and polarization properties of the beam using the technique of programmable diffractive optics. This technique is used facilitate active aberration correction in the beam and to generate radially polarized pupil function in order to get an on-axis axially polarized point spread function. We also develop the high numerical aperture theory to calculate the focal point spread function for a radially polarized pupil function. We describe the design and implementation of a simple vector beam formation unit consisting of a polarizing beam splitter and two right angled prisms in conjunction with a ferro-electric spatial light modulator. We also describe the design and implementation of a beam scanning system comprising of a novel off-axis scanner mirror that maintains registration of the conjugate pupil planes in the system.

Citation Download Citation

B. R. Boruah and M. A. A. Neil "Programmable diffractive optics for laser scanning confocal microscopy", Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 644310 (14 February 2007); https://doi.org/10.1117/12.700611

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