Erythrocytes volume and refractive index measurement with a digital holographic microscope

B. Rappaz; A. Barbul; F. Charrière; J. Kühn; P. Marquet; R. Korenstein; C. Depeursinge; P. Magistretti

doi:10.1117/12.700463

14 February 2007 Erythrocytes volume and refractive index measurement with a digital holographic microscope

B. Rappaz, A. Barbul, F. Charrière, J. Kühn, P. Marquet, R. Korenstein, C. Depeursinge, P. Magistretti

Proceedings Volume 6445, Optical Diagnostics and Sensing VII; 644509 (2007) https://doi.org/10.1117/12.700463
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We have recently proposed a new and efficient decoupling procedure allowing to directly obtain separate measurements of the thickness and the integral refractive index of a given living cell. Consequently, it has been possible to accurately measure (with a precision of 0.0003) the mean refractive index and the volume of living erythrocytes. Here, application of this decoupling procedure on erythrocyte allows to measure a refractive index of 1.40 and a mean volume of about 106 μm³.

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B. Rappaz, A. Barbul, F. Charrière, J. Kühn, P. Marquet, R. Korenstein, C. Depeursinge, and P. Magistretti "Erythrocytes volume and refractive index measurement with a digital holographic microscope", Proc. SPIE 6445, Optical Diagnostics and Sensing VII, 644509 (14 February 2007); https://doi.org/10.1117/12.700463

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