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Proceedings Article

Erythrocytes volume and refractive index measurement with a digital holographic microscope

[+] Author Affiliations
B. Rappaz

Brain Mind Institute, EPFL (Switzerland)

A. Barbul, R. Korenstein

Sackler Faculty of Medicine (Israel)

F. Charrière, J. Kühn, C. Depeursinge

Imaging and Applied Optics Institute, EPFL (Switzerland)

P. Marquet

Ctr. de Neurosciences Psychiatriques, DP-CHUV (Switzerland)

P. Magistretti

Brain Mind Institute, EPFL (Switzerland) and Ctr. de Neurosciences Psychiatriques, DP-CHUV (Switzerland)

Proc. SPIE 6445, Optical Diagnostics and Sensing VII, 644509 (February 14, 2007); doi:10.1117/12.700463
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From Conference Volume 6445

  • Optical Diagnostics and Sensing VII
  • Gerard L. Coté; Alexander V. Priezzhev
  • San Jose, CA | January 20, 2007

abstract

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We have recently proposed a new and efficient decoupling procedure allowing to directly obtain separate measurements of the thickness and the integral refractive index of a given living cell. Consequently, it has been possible to accurately measure (with a precision of 0.0003) the mean refractive index and the volume of living erythrocytes. Here, application of this decoupling procedure on erythrocyte allows to measure a refractive index of 1.40 and a mean volume of about 106 μm3.

© (2007) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

B. Rappaz ; A. Barbul ; F. Charrière ; J. Kühn ; P. Marquet, et al.
"Erythrocytes volume and refractive index measurement with a digital holographic microscope", Proc. SPIE 6445, Optical Diagnostics and Sensing VII, 644509 (February 14, 2007); doi:10.1117/12.700463; http://dx.doi.org/10.1117/12.700463


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