Spatial analysis of a microbead using surface plasmon resonance coupled fluorescence

Soon Joon Yoon; Donghyun Kim

doi:10.1117/12.700343

14 February 2007 Spatial analysis of a microbead using surface plasmon resonance coupled fluorescence

Soon Joon Yoon, Donghyun Kim

Proceedings Volume 6450, Plasmonics in Biology and Medicine IV; 64500O (2007) https://doi.org/10.1117/12.700343
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Surface plasmon resonance (SPR) coupled fluorescence uses an evanescent electromagnetic field to excite fluorophores in the vicinity of surface. We investigated the influence of enhanced evanescent fields at SPR on the induced fluorescence intensity. The system of this study is based on angle scanning with a half-cylinder prism (SF10) and dual motorized rotation stages to observe the correlation between the evanescent fields and fluorescent intensity of microbeads. With this system, emission from fluorophores only exists in close proximity to the surface of the microbead. The results show that evanescent fields produced at SPR provide more sensitive fluorescence images compared to those measured at a total internal reflection angle.

Citation Download Citation

Soon Joon Yoon and Donghyun Kim "Spatial analysis of a microbead using surface plasmon resonance coupled fluorescence", Proc. SPIE 6450, Plasmonics in Biology and Medicine IV, 64500O (14 February 2007); https://doi.org/10.1117/12.700343

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