The involvement of MAP kinases JNK and p38 in photodynamic injury of crayfish neurons and glial cells

Y. O. Petin; M. Y. Bibov; A. B. Uzdensky

doi:10.1117/12.741008

26 April 2007 The involvement of MAP kinases JNK and p38 in photodynamic injury of crayfish neurons and glial cells

Y. O. Petin, M. Y. Bibov, A. B. Uzdensky

Proceedings Volume 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII; 65351S (2007) https://doi.org/10.1117/12.741008
Event: Saratov Fall Meeting 2006, 2006, Saratov, Russian Federation

Abstract

The role of JNK and p38 MAP kinases in functional inactivation and necrosis of mechanoreceptor neurons as well as necrosis, apoptosis and proliferation of satellite glial cells induced by photodynamic treatment (10^-7 M Photosens, 30 min incubation, 670 nm laser irradiation at 0.4 W/cm²) in the isolated crayfish stretch receptor was studied using specific inhibitors SP600125 and SB202190, respectively. SP600125 enhanced PDT-induced apoptosis of photosensitized glial cells but did not influence PDT-induced changes in neuronal activity, density of glial nuclei around neuron body, and necrosis of receptor neurons and glial cells. SB202190 did not influence neuron activity and survival as well but reduced PDT-induced necrosis but not apoptosis of glial cells. Therefore, both MAP kinases influenced glial cells but not neurons. JNK protected glial cells from PDT-induced apoptosis but did not influence necrosis and proliferation of these cells. In contrast, p38 did not influence apoptosis but contributed into PDT-induced necrosis of glial cells and PDT-induced gliosis. These MAP kinase inhibitors may be used for modulation of photodynamic therapy of brain tumors.

Citation Download Citation

Y. O. Petin, M. Y. Bibov, and A. B. Uzdensky "The involvement of MAP kinases JNK and p38 in photodynamic injury of crayfish neurons and glial cells", Proc. SPIE 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII, 65351S (26 April 2007); https://doi.org/10.1117/12.741008

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