Photonic crystal biosensor microplates with integrated fluid networks for high throughput applications in drug discovery

Charles J. Choi; Leo L. Chan; Maria F. Pineda; Brian T. Cunningham

doi:10.1117/12.731324

11 September 2007 Photonic crystal biosensor microplates with integrated fluid networks for high throughput applications in drug discovery

Charles J. Choi, Leo L. Chan, Maria F. Pineda, Brian T. Cunningham

Author Affiliations +

Proceedings Volume 6645, Nanoengineering: Fabrication, Properties, Optics, and Devices IV; 66451H (2007) https://doi.org/10.1117/12.731324
Event: NanoScience + Engineering, 2007, San Diego, California, United States

Abstract

Assays used in pharmaceutical research require a system that can not only detect biochemical interactions with high sensitivity, but that can also perform many measurements in parallel while consuming low volumes of reagents. While nearly all label-free biosensor transducers to date have been interfaced with a flow channel, the liquid handling system is typically aligned and bonded to the transducer for supplying analytes to only a few sensors in parallel. In this presentation, we describe a fabrication approach for photonic crystal biosensors that utilizes nanoreplica molding to produce a network of sensors that are automatically self-aligned with a microfluidic network in a single process step. The sensor/fluid network is inexpensively produced on large surface areas upon flexible plastic substrates, allowing the device to be incorporated into standard format 96-well microplates. A simple flow scheme using hydrostatic pressure applied through a single control point enables immobilization of capture ligands upon a large number of sensors with 220 nL of reagent, and subsequent exposure of the sensors to test samples. A high resolution imaging detection instrument is capable of monitoring the binding within parallel channels at rates compatible with determining kinetic binding constants between the immobilized ligands and the analytes. The first implementation of this system is capable of monitoring the kinetic interactions of 11 flow channels at once, and a total of 88 channels within an integrated biosensor microplate in rapid succession. The system was initially tested to characterize the interaction between sets of proteins with known binding behavior.

Citation Download Citation

Charles J. Choi, Leo L. Chan, Maria F. Pineda, and Brian T. Cunningham "Photonic crystal biosensor microplates with integrated fluid networks for high throughput applications in drug discovery", Proc. SPIE 6645, Nanoengineering: Fabrication, Properties, Optics, and Devices IV, 66451H (11 September 2007); https://doi.org/10.1117/12.731324

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Sensors

Microfluidics

Biosensors

Biological research

Image sensors

Proteins

Photonic crystals

Show All Keywords

Keywords/Phrases

Search In:

Publication Years