We have been investigating ultrahigh resolution optical coherence tomography (UHR-OCT) imaging of lung tissues
using fiber super continuum sources. The high power, low-noise, Gaussian shaped supercontinuum generated with
ultrashort pulses and optical fibers at several wavelengths were used as the broadband light sources for UHR-OCT. For
the 800 nm wavelength region, the axial resolution was 3.0 um in air and 2.0 um in tissue. Since the lung consists of tiny
alveoli which are separated by thin wall, the UHR-OCT is supposed to be effective for lung imaging. The clear images of
alveoli of rat were observed with and without index matching effects by saline.
In this work, we investigated the UHR-OCT imaging of lung disease model. The lipopolysaccharide (LPS) induced acute
lung injury / acute respiratory distress syndrome (ALI/ARDS) model of rat was prepared as the sample with disease and
the UHR-OCT imaging of the disease part was demonstrated. The increment of signal intensity by pleural thickening
was observed. The accumulation of exudative fluid in alveoli was also observed for two samples. By the comparison
with normal lung images, we can obviously show the difference in the ALI/ARDS models.
Since the lung consists of alveolar surrounded by capillary vessels, the effect of red-blood cells (RBC) is considered to
be important. In this work, ex-vivo UHR-OCT imaging of RBC was demonstrated. Each RBC was able to be observed
individually using UHR-OCT. The effect of RBC was estimated with the rat lung perfused with PBS.© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.